Delineation of the discontinuous-conformational epitope of a monoclonal antibody displaying full in vitro and in vivo thyrotropin activity.

Title:

Delineation of the discontinuous-conformational epitope of a monoclonal antibody displaying full in vitro and in vivo thyrotropin activity.

identifier:	1713264
description:	epitope description:Q51, Q97 antigen name:Thyrotropin receptor host organism:Mus musculus BALB/c antibody name:IRI-SAb1
aggregation:	instance of dataset
availability:	available
primaryPublications:	15319453

study type:	b cell assays
subject species:	Homo sapiens

fullName:	Sabine Costagliola Marco Bonomi Nils G Morgenthaler Joost Van Durme Valé rie Panneels Samuel Refetoff Gilbert Vassart

method:	biological activity

name:	Delineation of the discontinuous-conformational epitope of a monoclonal antibody displaying full in vitro and in vivo thyrotropin activity.
description:	MAb IRI-SAb1, which recognizes the epitopic residues, bound to TSHR and weakly stimulated cAMP production in JP26 cells. This was only 10% of the maximum stimulation generated with bovine TSH, indicating that mAb acts as a partial agonist. MAb IRI-SAb1 Fab fragments also acted as partial agonists. MAb IRI-SAb1 inhibits the binding of bovine TSH to the TSH receptor in a dose-dependent manner.

name:	iedb
homePage:	http://www.iedb.org

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