Key residues contributing to dominant conformational autoantigenic epitopes on thyroid peroxidase identified by mutagenesis.

Title:

Key residues contributing to dominant conformational autoantigenic epitopes on thyroid peroxidase identified by mutagenesis.

identifier:	1713583
description:	epitope description:K627 antigen name:Thyroid peroxidase (UniProt:P07202) host organism:Homo sapiens antibody name:126TP5, 126TP14, 131TP7
aggregation:	instance of dataset
availability:	available
primaryPublications:	15240118

study type:	b cell assays
subject species:	Homo sapiens

fullName:	Monika Gora Andrzej Gardas Philip F Watson Paul Hobby Anthony P Weetman Brian J Sutton J Paul Banga

method:	ELISA

name:	Key residues contributing to dominant conformational autoantigenic epitopes on thyroid peroxidase identified by mutagenesis.
description:	Human monoclonal Fab fragments (rhFabs) specific for the immunodominant region B (IDR-B) bound to wild-type TPO and selected point mutants by ELISA. Single amino acid mutant K627A abolished binding for rhFabs 126TP5, 126TP14, and 131TP7, demonstrating this is a key residue in IDR-B. Point mutant Y772A also showed reduced binding, but the authors suggest that this mutation may effect the overall tertiary structure of TPO.

name:	iedb
homePage:	http://www.iedb.org

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