Structural insights into autoreactive determinants in thyroid peroxidase composed of discontinuous and multiple key contact amino acid residues contributing to epitopes recognized by patients' autoantibodies.

Title:

Dataset

identifier:	1713585
description:	epitope description:R225, R646, D707 antigen name:Thyroid peroxidase (UniProt:P07202) host organism:Homo sapiens antibody name:126TO10, 126TP1
aggregation:	instance of dataset
availability:	available
primaryPublications:	16959834

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1017349
landingPage:	http://www.iedb.org/assay/1713585

Publication

type:	Literature
publicationVenue:	Endocrinology
dates:	2006

Dimension

study type:	b cell assays
subject species:	Homo sapiens

Person

fullName:	Marlena Dubska J Paul Banga Danuta Plochocka Grazyna Hoser E Helen Kemp Brian J Sutton Andrzej Gardas Monika Gora

Data Type

method:	flow cytometry

Study

name:	Structural insights into autoreactive determinants in thyroid peroxidase composed of discontinuous and multiple key contact amino acid residues contributing to epitopes recognized by patients' autoantibodies.
description:	Human monoclonal Fab fragments (rhFabs) specific for the immunodominant region A (IDR-A) bound to wild-type TPO and selected point mutants by FACS. Triple mutant R225A/R646A/D707 reduced binding approximately 55-fold. Double mutants R225A/R646A, R225A/D707, or R646A/D707 reduced binding approximately 3- to 6-fold. The results were confirmed by ELISA, which showed greater sensitivity to the mutations with single mutations R225A, D707N and R646A showing significantly reduced binding The authors conclude that residues R225, R646, and D707 contribute to the IDR-A epitope.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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