Delineation of the discontinuous-conformational epitope of a monoclonal antibody displaying full in vitro and in vivo thyrotropin activity.

Title:

Delineation of the discontinuous-conformational epitope of a monoclonal antibody displaying full in vitro and in vivo thyrotropin activity.

identifier:	1713301
description:	epitope description:T62, K64, I66, R86, Y88, I91,H111, E113, R115 antigen name:Thyrotropin receptor host organism:Mus musculus NMRI
aggregation:	instance of dataset
availability:	available
primaryPublications:	15319453

study type:	b cell assays
subject species:	Homo sapiens

fullName:	Sabine Costagliola Marco Bonomi Nils G Morgenthaler Joost Van Durme Valé rie Panneels Samuel Refetoff Gilbert Vassart

method:	biological activity

name:	Delineation of the discontinuous-conformational epitope of a monoclonal antibody displaying full in vitro and in vivo thyrotropin activity.
description:	MAb IRI-SAb2, which recognizes the epitopic residues, increased total T4 thyroxine levels and decreased TSH levels in recipient mice compared to controls. Levels were altered as early as 8 h after injection and remained altered at 4 days. The T4 level returned to normal at 7 days, consistent with the serum half-life of of mouse IgG2a. Thyroid morphology showed hypertrophic cells in the follicular epithelial layers and necrotic thyrocytes in the follicular lumina. CD45+ immune cells and Mac-1+ macrophages were observed between the thyrocytes and inside the colloid. The mAb induced hyperthyroidism.

name:	iedb
homePage:	http://www.iedb.org

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