Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 1717857 |
| description: |
epitope description:I1738, T1739, T1740, I1741, D1742, P1744, W1745, N1746, D1772, F1776, R1779, M1785, H1786
antigen name:von Willebrand factor
host organism:Mus musculus
antibody name:82D6A3
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| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
16314412 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1002488 |
| landingPage: |
http://www.iedb.org/assay/1717857 |
| type: |
Literature
|
| publicationVenue: |
J Biol Chem
|
| dates: |
2006
|
| study type: | b cell assays |
| subject species: | Homo sapiens |
| fullName: |
Stephanie Staelens
Michael A Hadders
Stephan Vauterin
Cé
line Platteau
Marc De Maeyer
Karen Vanhoorelbeke
Eric G Huizinga
Hans Deckmyn
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| method: |
ELISA
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| name: |
Paratope determination of the antithrombotic antibody 82D6A3 based on the crystal structure of its complex with the von Willebrand factor A3-domain.
|
| description: |
Epitope-specific scFv 82D6A3 construct of 82D6A3 bound to vWF by ELISA. Mutants with single alanine substitutions of residues in 82D6A3 that form hydrogen bonds or salt bridges with the A3 domain of vWF showed that heavy chain residues N31, Y32, D99, P101, Y102 and Y103 had a strong effect on binding, heavy chain residue W50 and light chain residue H49 had a moderate effect, and heavy chain residue N54 and light chain residues Y32, P56, D92 and R95 had a minor or no effect.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
