Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1017942 |
| landingPage: |
http://www.iedb.org/assay/1762474 |
| type: |
Literature
|
| publicationVenue: |
Hybridoma
|
| dates: |
1995
|
| study type: | b cell assays |
| subject species: | Homo sapiens |
| fullName: |
W Klootwijk
L M Vaessen
B F Bernard
J M Rondeel
W J De Greef
T J Visser
|
| method: |
antigen inhibition
|
| name: |
Production and characterization of monoclonal and polyclonal antibodies against thyrotropin-releasing hormone.
|
| description: |
The amino terminal residue of thyrotropin-releasing hormone is pyrrolidine carboxylic acid and the C-terminal residue is proline amide.
The epitope displaced the binding of mAbs 83-7B5-A1 and 83-6B6-A10 to TRH-[125I]tyramine. Binding of mAb 83-7B5-A1 was inhibited by the epitope with or without amidation of the C-terminal proline residue. The longer peptides EHPG or EHPGK, both with N-terminal pyrrolidine carboxylic acid, partially inhibited binding.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
