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Title: Thermodynamic investigation of monoclonal antibody alkylated nucleoside interaction as a model for epitope recognition on nucleic acids.
identifier: 1800694
description:
epitope description:O(6)-methyl-2'-deoxyguanosine
host organism:Mus musculus BALB/c
antibody name:EM-1
aggregation:
instance of dataset
availability:
available
primaryPublications: 2441745
authorizations:
registration not required
accessURL: http://www.iedb.org/reference/1016377
landingPage: http://www.iedb.org/assay/1800694
type:
Literature
publicationVenue:
Biochemistry
dates:
1987
study type: b cell assays
subject species:
fullName:
S C Yuhasz
D F Senear
J Adamkiewicz
M F Rajewsky
P O Ts'o
L S Kan
method:
ELISA
name:
Thermodynamic investigation of monoclonal antibody alkylated nucleoside interaction as a model for epitope recognition on nucleic acids.
description:
The intrinsic binding constant was evaluated by nitrocellulose affinity filter assay (NAFA). The binding was better at pH 4, lower NaCl concentration, and best at higher termperatures.

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