Mutations of an antibody binding energy hot spot on domain III of the dengue 2 envelope glycoprotein exploited for neutralization escape.

Title:

Mutations of an antibody binding energy hot spot on domain III of the dengue 2 envelope glycoprotein exploited for neutralization escape.

identifier:	1804057
description:	epitope description:K307, K310, I312 antigen name:Genome polyprotein host organism:Mus musculus BALB/c antibody name:MAb 1A1D-2
aggregation:	instance of dataset
availability:	available
primaryPublications:	20832836

study type:	b cell assays
subject species:	Dengue virus 2 New Guinea C

fullName:	Gregory D Gromowski John T Roehrig Michael S Diamond J Ching Lee Trevor J Pitcher Alan D T Barrett

method:	biological activity

name:	Mutations of an antibody binding energy hot spot on domain III of the dengue 2 envelope glycoprotein exploited for neutralization escape.
description:	The ability of MAb 1A1D-2 to neutralize DENV infectivity was quantified using a plaque reduction neutralization test (PRNT). The PRNT50 concentration with DENV-2 strain NGC was 2.1±0.1 nM. In experiments to assess ED3 occupancy on the virion, the authors found that the majority of virus infectivity was neutralized at relatively high occupancy levels. Mutations at residues K307, K310 and I312 completely abrogated neutralization activity.

name:	iedb
homePage:	http://www.iedb.org

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