Crystal structure of the anti-His tag antibody 3D5 single-chain fragment complexed to its antigen.

Title:

Crystal structure of the anti-His tag antibody 3D5 single-chain fragment complexed to its antigen.

identifier:	1808701
description:	epitope description:HHHHHH host organism:Mus musculus C57BL/6 antibody name:anti-His 3D5 (mut1+2)
aggregation:	instance of dataset
availability:	available
primaryPublications:	12054774

study type:	b cell assays
subject species:

fullName:	Markus Kaufmann Peter Lindner Annemarie Honegger Kerstin Blank Markus Tschopp Guido Capitani Andreas Plü ckthun Markus G Grü tter

method:	western blot

name:	Crystal structure of the anti-His tag antibody 3D5 single-chain fragment complexed to its antigen.
description:	The epitope is a widely used His tag, consisting of 6 consecutive histidine residues. Epitope specific 3D5 scFv (mut1+2) recognized a mouse scFv fragment (anti-HER2 antibody 4D5) with a His tag of three, four or six histidine residues at the C-terminus. It did not recognize a scFv with six histidine residues followed by a glycine residue. It was concluded that 3D5 recognizes 3 histidine residues and the last residue must be histidine.

name:	iedb
homePage:	http://www.iedb.org

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