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identifier: 1819661
description:
epitope description:X: E18, P19, S20, T55, L56, S57, D58, N60, I61, Q62; Y: L8, T9, E34, G35, I36, P37, D39, Q40, L71, R72, L73, R74, G75, G76
antigen name:Ubiquitin-40S ribosomal protein S27a (UniProt:P62979)
host organism:Homo sapiens
antibody name:Apu3.A8
aggregation:
instance of dataset
availability:
available
primaryPublications: 18724939
authorizations:
registration not required
accessURL: http://www.iedb.org/reference/1019698
landingPage: http://www.iedb.org/assay/1819661
type:
Literature
publicationVenue:
Cell
dates:
2008
study type: b cell assays
subject species: Homo sapiens
fullName:
Kim Newton
Marissa L Matsumoto
Ingrid E Wertz
Donald S Kirkpatrick
Jennie R Lill
Jenille Tan
Debra Dugger
Nathaniel Gordon
Sachdev S Sidhu
Frederic A Fellouse
Laszlo Komuves
Dorothy M French
Ronald E Ferrando
Cynthia Lam
Deanne Compaan
Christine Yu
Ivan Bosanac
Sarah G Hymowitz
Robert F Kelley
Vishva M Dixit
method:
immunoprecipitation
name:
Ubiquitin chain editing revealed by polyubiquitin linkage-specific antibodies.
description:
The epitope residues are calculated from the PDB structure [PDB: 3DVG] as the antigen residues at 4 Å
distance from the antibody.
Epitope specific Apu3.A8 immunoprecipitated ubiquitinated TRAF6 protein from cell lysates of human embryonic kidney 293T cells, ubiquitinated IRAK1 from IL-1ß-treated 293T cells, ubiquitinated RIP1 from TNF-treated HeLa cells, and other polyubiquitinated proteins from human BJAB cells.

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