Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1020644 |
| landingPage: |
http://www.iedb.org/assay/1821095 |
| type: |
Literature
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| publicationVenue: |
Xenotransplantation
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| dates: |
2003
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| study type: | b cell assays |
| subject species: | Sus scrofa |
| fullName: |
Jining Liu
Andrej Weintraub
Jan Holgersson
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| method: |
antigen inhibition
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| name: |
Multivalent Galalpha1,3Gal-substitution makes recombinant mucin-immunoglobulins efficient absorbers of anti-pig antibodies.
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| description: |
The epitope inhibited cytoxocity of human sera against porcine aortic endothelian cells (PEC-A). The epitope was expressed on the extracellular portion of a cell surface-anchored mucin, P-selectin glycoprotein ligand-1 (PSGL-1) fused to an immunoglobulin Fc portion to form the PSGL1/mIgG2b construct. Absorption of human sera with PSGL1/mIgG2b inhibited complement-dependent cytotoxicity against PEC-A and depleted reactivity with all membrane components of PAC-A cells. The decrease in cytoxicity correlated with the decrease in reactivity to PAC-A cell components. The inhibition was greater with the PSGL1/mIgG2b construct compared to the epitope conjugated to agarose beads or macroporous glass.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
