Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 1821941 |
| description: |
epitope description:R108, N109, V110, G111, S112, Q113, L115, N180, I185, N189, S236, S237, R239, P240, G241, I242, L244, W245, H246
antigen name:Integrin alpha-IIb
host organism:Mus musculus BALB/c
antibody name:10E5
|
| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
18710925 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1019694 |
| landingPage: |
http://www.iedb.org/assay/1821941 |
| type: |
Literature
|
| publicationVenue: |
J Cell Biol
|
| dates: |
2008
|
| study type: | b cell assays |
| subject species: | Homo sapiens |
| fullName: |
Timothy A Springer
Jianghai Zhu
Tsan Xiao
|
| method: |
x-ray crystallography
|
| name: |
Structural basis for distinctive recognition of fibrinogen gammaC peptide by the platelet integrin alphaIIbbeta3.
|
| description: |
The epitope residues are calculated from the PDB structures as the antigen residues at 4 Å
distance from the antibody.
The epitope of Fab 10E5 on integrin alphaIIbß3 was determined from the crystal structure of the complex. Fab 10E5 binds solely to the alphaIIb cap subdomain and has no effect on its conformation. The peptide LGGAKQRGDV was soaked into crystals of the soluble headpiece of integrin alphaIIbbeta3 (chain A: Integrin alphaIIb chain B: Integrin beta3) and the 10E5 Fab. Also, other structures were obtained by soaking other peptides, 2VDP, 2VDQ, 2VDO, 2VDN, and without peptides, 2VC2, 2VDK, 2VDL, 2VDM. Calculations show that the epitopes were identical in all structures; therefore, only one structure with the best resolution was curated.
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| name: |
iedb
|
| homePage: |
http://www.iedb.org |
