Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 1823568 |
| description: |
epitope description:A: S570, R573, V576; B: D754, D828, R855, N857, Q858, E859, K861, P890, H918, H919, F920, I921
antigen name:Complement C3
host organism:Homo sapiens
antibody name:S77
|
| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
19196712 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1019637 |
| landingPage: |
http://www.iedb.org/assay/1823568 |
| type: |
Literature
|
| publicationVenue: |
J Biol Chem
|
| dates: |
2009
|
| study type: | b cell assays |
| subject species: | Homo sapiens |
| fullName: |
Kenneth J Katschke Jr
Scott Stawicki
Jianping Yin
Micah Steffek
Hongkang Xi
Lizette Sturgeon
Philip E Hass
Kelly M Loyet
Laura Deforge
Yan Wu
Menno van Lookeren Campagne
Christian Wiesmann
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| method: |
surface plasmon resonance (SPR)
|
| name: |
Structural and functional analysis of a C3b-specific antibody that selectively inhibits the alternative pathway of complement.
|
| description: |
The epitope residues are calculated from [PDB: 3G6J] at 4 Å
distance. Epitope is located on alpha and beta-chains of C3b. PDB chain B is the C3b alpha-chain, PDB chain A is the beta-chain. Epitope residue on beta-chain in GenBank numbering [GI:119370332] are S570, R573, V576
on alpha-chain (same GI number) are D754, D828, R855, N857, Q858, E859, K861, P890, H918, H919, F920, I921.
The epitope specific S77 Fab bound to complement C3b with high affinity and bound to hydrolyzed C3 (iC3; KD = 1.2 nM), iC3b (KD = 2.0 nM) and C3c (KD = 2.2 nM). S77 did not bind significantly to C3 by ELISA.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
