Immunological Data Discovery Index
Immunological Data Discovery Index
| Title: | Functional intracellular antibody fragments do not require invariant intra-domain disulfide bonds. |
| identifier: | 1832999 |
| description: |
epitope description:S17, Q25, H27, V29, E31, Y32, D33, P34, T35, I36, E37, D38, S39, Y40, Q61, E63, Y64
antigen name:GTPase HRas
host organism:Homo sapiens
antibody name:HRAS-Fv, disulfide-free anti-RAS, VH#6(AV) and VL#204(VA)
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| aggregation: |
instance of dataset
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| availability: |
available
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| primaryPublications: |
18187153 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1018819 |
| landingPage: |
http://www.iedb.org/assay/1832999 |
| type: |
Literature
|
| publicationVenue: |
J Mol Biol
|
| dates: |
2008
|
| study type: | b cell assays |
| subject species: | Homo sapiens |
| fullName: |
Tomoyuki Tanaka
Terence H Rabbitts
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| method: |
biological activity
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| name: |
Functional intracellular antibody fragments do not require invariant intra-domain disulfide bonds.
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| description: |
Epitope residues were calculated from the structure [PDB: 2VH5] as the antigen residues interacting with the antibody fragment at atomic distance of 4 Å
.
The epitope specific disulfide-free anti-RAS, VH#6(AV) and VL#204(VA) (designated HRAS-Fv SS-free) reverted RAS-dependent transformation of NIH3T3-EJ cells as shown using an achorage independence assay. Expression of disulfide-free anti-RAS VH#6(AV) in NIH3T3-EJ cells inhibited soft agar colony formation. Expression of VH#6(AV) in NIH3T3-EJ cells also inhibited lung tumor development in vivo, assayed 14 days after injection of the cells.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |