Structural basis for the preferential recognition of immature flaviviruses by a fusion-loop antibody.

Title:

Structural basis for the preferential recognition of immature flaviviruses by a fusion-loop antibody.

identifier:	1831632
description:	epitope description:T76, G106 antigen name:Genome polyprotein host organism:Mus musculus BALB/c antibody name:E53
aggregation:	instance of dataset
availability:	available
primaryPublications:	19713934

study type:	b cell assays
subject species:	Dengue virus 2

fullName:	Mickaë l V Cherrier Bä rbel Kaufmann Grant E Nybakken Shee-Mei Lok Julia T Warren Beverly R Chen Christopher A Nelson Victor A Kostyuchenko Heather A Holdaway Paul R Chipman Richard J Kuhn Michael S Diamond Michael G Rossmann Daved H Fremont

method:	electron microscopy

name:	Structural basis for the preferential recognition of immature flaviviruses by a fusion-loop antibody.
description:	The entire sequence of the E53 epitope is conserved between WNV and all four DENV serotypes with the exception of residue M77, which is a glutamine in DENV. Epitope residues were calculated based on 4 Å cut-off atomic distance from [PDB: 3IXY]. WNV E-specific E53 Fab cross-recognized immature Dengue virus particles, but not mature Dengue virus. The epitope of E53 Fab on immature DENV particles was determined by fitting the atomic coordinates of E53 Fab and DENV E into the cryoEM density.

name:	iedb
homePage:	http://www.iedb.org

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