Structure and functional implications of the polymerase active site region in a complex of HIV-1 RT with a double-stranded DNA template-primer and an antibody Fab fragment at 2.8 A resolution.

Title:

Structure and functional implications of the polymerase active site region in a complex of HIV-1 RT with a double-stranded DNA template-primer and an antibody Fab fragment at 2.8 A resolution.

Dataset

identifier:	1838721
description:	epitope description:R199, K223, E224, P225, P226, F227, W229, M230, R358 antigen name:Gag-Pol polyprotein host organism:Mus musculus BALB/c antibody name:28
aggregation:	instance of dataset
availability:	available
primaryPublications:	9837729

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1093
landingPage:	http://www.iedb.org/assay/1838721

Publication

type:	Literature
publicationVenue:	J Mol Biol
dates:	1998

Dimension

study type:	b cell assays
subject species:	Human immunodeficiency virus 1

Person

fullName:	J Ding K Das Y Hsiou S G Sarafianos A D Clark Jr A Jacobo-Molina C Tantillo S H Hughes E Arnold

Data Type

method:	x-ray crystallography

Study

name:	Structure and functional implications of the polymerase active site region in a complex of HIV-1 RT with a double-stranded DNA template-primer and an antibody Fab fragment at 2.8 A resolution.
description:	The epitope residues are calculated from the PDB structure [PDB: 2HMI] as the antigen residues at 4 Å distance from the antibody. The epitope of Fab28 on the HIV-1 RT p51 subunit was determined from the crystal structure of Fab28 bound to the complex of HIV-1 RT p51/p66 dimer/dsDNA (19-mer/18-mer). Fab28 binds to the p51 palm subdomain and has no contacts with either the p66 subunit or the dsDNA. The HIV-1 RT p51 subunit contained the C280S mutation.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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