Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 1846597 |
| description: |
epitope description:T68, I127, R131, I135, T136, P138, F139, V222, A226, K267, E268, A271, T274, L275, I278, I325, Y326, R328, P330
antigen name:Beta-2 adrenergic receptor
host organism:Lama glama
antibody name:Nb80
|
| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
21228869 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1022177 |
| landingPage: |
http://www.iedb.org/assay/1846597 |
| type: |
Literature
|
| publicationVenue: |
Nature
|
| dates: |
2011
|
| study type: | b cell assays |
| subject species: | Homo sapiens |
| fullName: |
Sø
ren G F Rasmussen
Hee-Jung Choi
Juan Jose Fung
Els Pardon
Paola Casarosa
Pil Seok Chae
Brian T Devree
Daniel M Rosenbaum
Foon Sun Thian
Tong Sun Kobilka
Andreas Schnapp
Ingo Konetzki
Roger K Sunahara
Samuel H Gellman
Alexander Pautsch
Jan Steyaert
William I Weis
Brian K Kobilka
|
| method: |
biological activity
|
| name: |
Structure of a nanobody-stabilized active state of the beta(2) adrenoceptor.
|
| description: |
The epitope residues are calculated from the PDB structure [PDB: 3P0G] as the antigen residues at 4 Å
distance from the antibody.
Binding of epitope-specific nanobody Nb80 to the ß2AR-T4 lysozyme fusion protein, reconstituted into HDL particles, increased the affinity of the agonist isoproterenol for ß2AR-T4L, similar to the increase observed in the Gs-coupled state. Nb80 did not affect ß2AR-T4L binding to the inverse agonist ICI-118551. In the fusion protein, the third intracellular loop of ß2AR was replaced by T4 lysozyme.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
