Crystal structure of HIV-1 reverse transcriptase in complex with a polypurine tract RNA:DNA. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Crystal structure of HIV-1 reverse transcriptase in complex with a polypurine tract RNA:DNA.

Dataset

identifier:	1848480
description:	epitope description:R199, Q222, K223, E224, P225, P226, F227, W229, M230 antigen name:Gag-Pol polyprotein host organism:Mus musculus BALB/c antibody name:Fab28
aggregation:	instance of dataset
availability:	available
primaryPublications:	11250910

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1019667
landingPage:	http://www.iedb.org/assay/1848480

Publication

type:	Literature
publicationVenue:	EMBO J
dates:	2001

Dimension

study type:	b cell assays
subject species:	Human immunodeficiency virus 1

Person

fullName:	S G Sarafianos K Das C Tantillo A D Clark Jr J Ding J M Whitcomb P L Boyer S H Hughes E Arnold

Data Type

method:	x-ray crystallography

Study

name:	Crystal structure of HIV-1 reverse transcriptase in complex with a polypurine tract RNA:DNA.
description:	Epitope residues are calculated from the PDB structure [PDB: 1HYS] as the antigen residues at 4 Å atomic distance from the antibody. The epitope of Fab28 on the HIV-1 RT p51 subunit was determined from the crystal structure of Fab28 bound to HIV-1 RT p51/p66 heterodimer in complex with a polypurine tract containing RNA:DNA oligonucleotide. Fab28 binds to the p51 palm subdomain and has no contacts with either the p66 subunit or the RNA:DNA.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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