Mapping the binding of synthetic disaccharides representing epitopes of chlamydial lipopolysaccharide to antibodies with NMR.

Title:

Mapping the binding of synthetic disaccharides representing epitopes of chlamydial lipopolysaccharide to antibodies with NMR.

identifier:	1851763
description:	epitope description:alpha-Kdo-(2->8)-alpha-Kdo antigen name:lipopolysaccharide host organism:Mus musculus BALB/c antibody name:S23-24
aggregation:	instance of dataset
availability:	available
primaryPublications:	11041842

study type:	b cell assays
subject species:	Chlamydiae

fullName:	H Maaheimo P Kosma L Brade H Brade

method:	nuclear magnetic resonance (NMR)

name:	Mapping the binding of synthetic disaccharides representing epitopes of chlamydial lipopolysaccharide to antibodies with NMR.
description:	The mAb was purified by affinity chromatography using as immobilized ligand Kdo-(2-4)-Kdo-(2-6)-a-GlcNAc-(1-6)-a-GlcNAc 1,4'-P₂. A detailed analysis of the reactivity to the epitope-allyl structure was done by NMR techniques such as trNOESY, QUIET-trNOESY, STD NMR and STD TOCSY NMR experiments. It was shown that the most important part for binding to the mAb was the nonreducing Kdo unit.

name:	iedb
homePage:	http://www.iedb.org

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on Feedback.