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Title: Epitope analysis of monoclonal antibody E5/G6, which binds to a linear epitope in the nucleocapsid protein of hantaviruses.
identifier: 1853924
description:
epitope description:YEDVNGIRK
antigen name:Seoul orthohantavirus protein
host organism:Mus musculus BALB/c
antibody name:E5/G6
aggregation:
instance of dataset
availability:
available
primaryPublications: 15338326
authorizations:
registration not required
accessURL: http://www.iedb.org/reference/459
landingPage: http://www.iedb.org/assay/1853924
type:
Literature
publicationVenue:
Arch Virol
dates:
2004
study type: b cell assays
subject species: Seoul virus SR11
fullName:
M Okumura
K Yoshimatsu
K Araki
B-H Lee
A Asano
J Arikawa
method:
western blot
name:
Epitope analysis of monoclonal antibody E5/G6, which binds to a linear epitope in the nucleocapsid protein of hantaviruses.
description:
The epitope sequence was identified based on the SEOV sequence, but common to hantavirus nucleocapsid proteins and is also present in BCCV.
The epitope was identified as the most effective minimum epitope for E5/G6 mAb binding by using thirty two 10-residue peptides (between NP140 and 180 of SEO/SR-11, 9 residue overlapping) and different truncates between residue 165 Try and residue 174 Pro. Residues D167, G170, I171, and R172 were indispensable residues, and Y165, E166, K173, and P174 were necessary for an effective reaction but not indispensable.

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