Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 1852738 |
| description: |
epitope description:Y44, K45, K87, R123, T125, E126, D131, G132, H133, H134, S135, E136, D147, T149, R153, R155, Y174, E176, S177, K178, A179, H180
antigen name:Sonic hedgehog protein
host organism:Mus musculus
antibody name:ch5E1
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| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
20504762 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1022197 |
| landingPage: |
http://www.iedb.org/assay/1852738 |
| type: |
Literature
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| publicationVenue: |
J Biol Chem
|
| dates: |
2010
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| study type: | b cell assays |
| subject species: | Homo sapiens |
| fullName: |
Henry R Maun
Xiaohui Wen
Andreas Lingel
Frederic J de Sauvage
Robert A Lazarus
Suzie J Scales
Sarah G Hymowitz
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| method: |
bio-layer interferometry assay
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| name: |
Hedgehog pathway antagonist 5E1 binds hedgehog at the pseudo-active site.
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| description: |
Epitope residues are calculated from the structure [PDB: 3MXW] as the antigen residues at 4 Å
distance from the antibody.
The epitope specific Fab ch5E1 bound to Sonic hedgehog (Shh) with an affinity of 6.94 nM, as measured by biolayer interferometry. Addition of Ca2+ improved the affinity to 1.12 nM and Ca2+ plus Zn2+ further improved the affinity to 0.31 nM. The increase in affinity was mainly due to a decrease in the dissociation rate constant.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
