Epitope characterization and crystal structure of GA101 provide insights into the molecular basis for type I/II distinction of CD20 antibodies.

Title:

Epitope characterization and crystal structure of GA101 provide insights into the molecular basis for type I/II distinction of CD20 antibodies.

identifier:	1859296
description:	epitope description:ANPSEKNSP antigen name:B-lymphocyte antigen CD20 host organism:Mus musculus antibody name:GA101, B-Ly1, H299/B1
aggregation:	instance of dataset
availability:	available
primaryPublications:	21444918

study type:	b cell assays
subject species:	Homo sapiens

fullName:	Gerhard Niederfellner Alfred Lammens Olaf Mundigl Guy J Georges Wolfgang Schaefer Manfred Schwaiger Andreas Franke Kornelius Wiechmann Stefan Jenewein Jerry W Slootstra Peter Timmerman Annika Brä nnströ m Frida Lindstrom Ekkehard Mö ssner Pablo Umana Karl-Peter Hopfner Christian Klein

method:	ELISA

name:	Epitope characterization and crystal structure of GA101 provide insights into the molecular basis for type I/II distinction of CD20 antibodies.
description:	The epitope footprint of the epitope-reactive GA101 mAb was determined by pepscan of overlapping adjacent 8-mer peptides spanning the sequence of the CD20 large extracellular loop, residues 142-187 (with Cys to Ala substitutions). The footprint of GA101, the parent mouse mAb B-Ly1, and an unrelated type II anti-CD20 mAb H299/B1, started around position Ala167 and extended to Pro178. Single alanine substitutions demonstrated that the core epitope of GA101 was formed by residues 172-178 (PSEKNSP).

name:	iedb
homePage:	http://www.iedb.org

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