Epitope characterization and crystal structure of GA101 provide insights into the molecular basis for type I/II distinction of CD20 antibodies.

Title:

Epitope characterization and crystal structure of GA101 provide insights into the molecular basis for type I/II distinction of CD20 antibodies.

identifier:	1859299
description:	epitope description:ANPS antigen name:B-lymphocyte antigen CD20 host organism:Mus musculus BALB/c antibody name:Rituximab, C2H7, LT20
aggregation:	instance of dataset
availability:	available
primaryPublications:	21444918

study type:	b cell assays
subject species:	Homo sapiens

fullName:	Gerhard Niederfellner Alfred Lammens Olaf Mundigl Guy J Georges Wolfgang Schaefer Manfred Schwaiger Andreas Franke Kornelius Wiechmann Stefan Jenewein Jerry W Slootstra Peter Timmerman Annika Brä nnströ m Frida Lindstrom Ekkehard Mö ssner Pablo Umana Karl-Peter Hopfner Christian Klein

method:	ELISA

name:	Epitope characterization and crystal structure of GA101 provide insights into the molecular basis for type I/II distinction of CD20 antibodies.
description:	The epitope footprint of the epitope-specific Rituximab mAb was determined by pepscan of overlapping adjacent 8-mer peptides spanning the sequence of the CD20 large extracellular loop, residues 142-187 (with Cys to Ala substitutions). The footprint of Rituximab, and the unrelated type I anti-CD20 mAbs C2H7 and LT20, started around position Ile162 and extended to Asn176. Single alanine substitutions demonstrated that the core epitope of Rituximab was formed by residues 168-175.

name:	iedb
homePage:	http://www.iedb.org

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