The structural basis of repertoire shift in an immune response to phosphocholine. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

The structural basis of repertoire shift in an immune response to phosphocholine.

Dataset

identifier:	1898703
description:	epitope description:p-nitrophenylphosphocholine host organism:Mus musculus BALB/c antibody name:M3C65
aggregation:	instance of dataset
availability:	available
primaryPublications:	10859335

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1010945
landingPage:	http://www.iedb.org/assay/1898703

Publication

type:	Literature
publicationVenue:	J Exp Med
dates:	2000

Dimension

study type:	b cell assays
subject species:

Person

fullName:	M Brown M A Schumacher G D Wiens R G Brennan M B Rittenberg

Data Type

method:	quenching

Study

name:	The structural basis of repertoire shift in an immune response to phosphocholine.
description:	The antibody used was mutated at residues N52K, N53H and A55T of the light chain, which resulted in increased affinity for the epitope compared to the germline antibody. Binding by variants of the epitope-specific monoclonal antibody was also measured. The sFv measured 2.8 x 10^-3 nM^-1 and the germline antibody measured 3 x 10^-5 nM^-1.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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