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Title: The crystal structure of sphingosine-1-phosphate in complex with a Fab fragment reveals metal bridging of an antibody and its antigen.
identifier: 1882560
description:
epitope description:sphingosine 1-phosphate
host organism:Mus musculus
antibody name:LT1009
aggregation:
instance of dataset
availability:
available
primaryPublications: 19815502
authorizations:
registration not required
accessURL: http://www.iedb.org/reference/1019703
landingPage: http://www.iedb.org/assay/1882560
type:
Literature
publicationVenue:
Proc Natl Acad Sci U S A
dates:
2009
study type: b cell assays
subject species:
fullName:
Jonathan M Wojciak
Norman Zhu
Karen T Schuerenberg
Kelli Moreno
William S Shestowsky
Masao Hiraiwa
Roger Sabbadini
Tom Huxford
method:
surface plasmon resonance (SPR)
name:
The crystal structure of sphingosine-1-phosphate in complex with a Fab fragment reveals metal bridging of an antibody and its antigen.
description:
Binding of the epitope-specific Fab to the epitope was measured by SPR. Adding 50 µM CaCl2 to the assay gave a kd of 2.9 x 10-4 s-1.

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