The interplay between binding energy and catalysis in the evolution of a catalytic antibody. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

The interplay between binding energy and catalysis in the evolution of a catalytic antibody.

Dataset

identifier:	1903710
description:	epitope description:(1S,2S,5S)-2-(4-glutaridylbenzyl)-5-phenylcyclohexan-1-ol host organism:Mus musculus antibody name:AZ-28
aggregation:	instance of dataset
availability:	available
primaryPublications:	9305839

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1019339
landingPage:	http://www.iedb.org/assay/1903710

Publication

type:	Literature
publicationVenue:	Nature
dates:	1997

Dimension

study type:	b cell assays
subject species:

Person

fullName:	H D Ulrich E Mundorff B D Santarsiero E M Driggers R C Stevens P G Schultz

Data Type

method:	surface plasmon resonance (SPR)

Study

name:	The interplay between binding energy and catalysis in the evolution of a catalytic antibody.
description:	The affinity of the catalytic antibody AZ-28 Fab for the epitope was determined by surface plasmon resonance. An antibody construct with the candidate germline variable regions bound the epitope with KD =670 nM. Introduction of somatic mutations into the germline heavy or light chain regions showed that there was an inverse correlation between the improved affinity during maturation and the catalytic rate for the oxy-cope rearrangement with residue Ser34 on the light chain being critical for the enhanced catalytic rate.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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