Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1096 |
| landingPage: |
http://www.iedb.org/assay/1910999 |
| type: |
Literature
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| publicationVenue: |
Proc Natl Acad Sci U S A
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| dates: |
1999
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| study type: | b cell assays |
| subject species: | Human immunodeficiency virus 1 |
| fullName: |
S G Sarafianos
K Das
A D Clark Jr
J Ding
P L Boyer
S H Hughes
E Arnold
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| method: |
x-ray crystallography
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| name: |
Lamivudine (3TC) resistance in HIV-1 reverse transcriptase involves steric hindrance with beta-branched amino acids.
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| description: |
Epitope residues are calculated from the structure [PDB: 1C9R] as the antigen residues at 4 Å
distance from the antibody.
The epitope of Fab 28 on the HIV-1 RT p51 subunit was determined from the crystal structure of Fab 28 bound to the complex of HIV-1 RT p51/p66 dimer/dsDNA (19-mer/18-mer). Fab 28 binds to the p51 palm subdomain and has no contacts with either the p66 subunit or the dsDNA. The HIV-1 RT p66 subunit contained the M184I mutation in the polymerase active site. Both RT subunits contained the C280S mutation.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
