| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1022739 |
| landingPage: |
http://www.iedb.org/assay/1911069 |
| type: |
Literature
|
| publicationVenue: |
PLoS Negl Trop Dis
|
| dates: |
2012
|
| study type: | b cell assays |
| subject species: | Dengue virus type 1 Hawaii |
| fullName: |
Hong-En Lin
Wen-Yang Tsai
I-Ju Liu
Pi-Chun Li
Mei-Ying Liao
Jih-Jin Tsai
Yi-Chieh Wu
Chih-Yun Lai
Chih-Hsuan Lu
Jyh-Hsiung Huang
Gwong-Jen Chang
Han-Chung Wu
Wei-Kung Wang
|
| method: |
western blot
|
| name: |
Analysis of epitopes on dengue virus envelope protein recognized by monoclonal antibodies and polyclonal human sera by a high throughput assay.
|
| description: |
Site-directed mutagenesis was performed on a DENV1 prM/E expression construct, pCB-D1, to replace each of the 67 E residues. Dot blot assays using these as antigens expressed by 293T cells were used for epitope mapping of human sera.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |