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identifier: 1911069
description:
epitope description:W101, L107
antigen name:Genome polyprotein
host organism:Homo sapiens
aggregation:
instance of dataset
availability:
available
primaryPublications: 22235356
authorizations:
registration not required
accessURL: http://www.iedb.org/reference/1022739
landingPage: http://www.iedb.org/assay/1911069
type:
Literature
publicationVenue:
PLoS Negl Trop Dis
dates:
2012
study type: b cell assays
subject species: Dengue virus type 1 Hawaii
fullName:
Hong-En Lin
Wen-Yang Tsai
I-Ju Liu
Pi-Chun Li
Mei-Ying Liao
Jih-Jin Tsai
Yi-Chieh Wu
Chih-Yun Lai
Chih-Hsuan Lu
Jyh-Hsiung Huang
Gwong-Jen Chang
Han-Chung Wu
Wei-Kung Wang
method:
western blot
name:
Analysis of epitopes on dengue virus envelope protein recognized by monoclonal antibodies and polyclonal human sera by a high throughput assay.
description:
Site-directed mutagenesis was performed on a DENV1 prM/E expression construct, pCB-D1, to replace each of the 67 E residues. Dot blot assays using these as antigens expressed by 293T cells were used for epitope mapping of human sera.

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