Antibody 2G12 recognizes di-mannose equivalently in domain- and nondomain-exchanged forms but only binds the HIV-1 glycan shield if domain exchanged.

Title:

Antibody 2G12 recognizes di-mannose equivalently in domain- and nondomain-exchanged forms but only binds the HIV-1 glycan shield if domain exchanged.

Dataset

identifier:	1917127
description:	epitope description:alpha-D-Manp-(1->2)-alpha-D-Manp host organism:Homo sapiens antibody name:2G12 I19R
aggregation:	instance of dataset
availability:	available
primaryPublications:	20702629

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1022154
landingPage:	http://www.iedb.org/assay/1917127

Publication

type:	Literature
publicationVenue:	J Virol
dates:	2010

Dimension

study type:	b cell assays
subject species:

Person

fullName:	Katie J Doores Zara Fulton Michael Huber Ian A Wilson Dennis R Burton

Data Type

method:	ELISA

Study

name:	Antibody 2G12 recognizes di-mannose equivalently in domain- and nondomain-exchanged forms but only binds the HIV-1 glycan shield if domain exchanged.
description:	The epitope-specific 2G12 I19R variant antibody is only able to bind the gp120 when initially pre-complexed with an anti-Human-Fc antibody to enhance the avidity of the interaction. The monovalent Fab fragment did not show significant binding to gp120. A dissociation constant could not be estimated by surface plasmon resonance. The complexed or uncomplexed 2G12 I19R variant antibody did not neutralize the HIV-1 JR-FL or JR-CSF pseudovirus.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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