Structural basis of HIV-1 resistance to AZT by excision. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Structural basis of HIV-1 resistance to AZT by excision.

Dataset

identifier:	1922176
description:	epitope description:R199, Q222, K223, E224, P225, P226, F227, L228, W229, M230, R358 antigen name:Gag-Pol polyprotein host organism:Mus musculus BALB/c antibody name:28
aggregation:	instance of dataset
availability:	available
primaryPublications:	20852643

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1022186
landingPage:	http://www.iedb.org/assay/1922176

Publication

type:	Literature
publicationVenue:	Nat Struct Mol Biol
dates:	2010

Dimension

study type:	b cell assays
subject species:	Human immunodeficiency virus 1

Person

fullName:	Xiongying Tu Kalyan Das Qianwei Han Joseph D Bauman Arthur D Clark Jr Xiaorong Hou Yulia V Frenkel Barbara L Gaffney Roger A Jones Paul L Boyer Stephen H Hughes Stefan G Sarafianos Eddy Arnold

Data Type

method:	x-ray crystallography

Study

name:	Structural basis of HIV-1 resistance to AZT by excision.
description:	Epitope residues are calculated from the structure [PDB: 3KLH] as the antigen residues at 4 Å distance from the antibody. The epitope of Fab28 on the HIV-1 RT p51 subunit was determined from the crystal structure of Fab28 bound to the complex of HIV-1 RT p51/p66 heterodimer/dsDNA (27-mer/21-mer). Fab28 binds to the p51 palm subdomain and has no contacts with either the p66 subunit or the dsDNA. The HIV-1 RT p51 subunit contained the C280S mutation and was truncated at Q428 with an added His tag. The RT p66 (AZTr) subunit contained the five excision-mediated AZT resistance mutations M41L, D67N, K70R, T215Y and K219Q, along with C280S and Q258C.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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