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Title: Covalent binding of nitroso-sulfonamides to glutathione S-transferase in guinea pigs with delayed type hypersensitivity.
identifier: 1931126
description:
epitope description:4-nitrosobenzenesulfonamide
host organism:Cavia porcellus Hartley
aggregation:
instance of dataset
availability:
available
primaryPublications: 22342371
authorizations:
registration not required
accessURL: http://www.iedb.org/reference/1022981
landingPage: http://www.iedb.org/assay/1931126
type:
Literature
publicationVenue:
Int Immunopharmacol
dates:
2012
study type: b cell assays
subject species:
fullName:
Reiko Eyanagi
Akihisa Toda
Masumi Imoto
Hidemori Uchiyama
Yuji Ishii
Hiroaki Kuroki
Yukako Kuramoto
Shinji Soeda
Hiroshi Shimeno
method:
ELISA
name:
Covalent binding of nitroso-sulfonamides to glutathione S-transferase in guinea pigs with delayed type hypersensitivity.
description:
Sera from guinea pigs immunized with the epitope showed a positive IgG reaction to experimentally generated conjugates of the epitope with guinea pig GST. More importantly, purified GST from the liver cells cytosol from the immunized animals was as good as antigen as the experimentally generated epitope-GST conjugate, confirming in vivo conjugation of the epitope with liver GST in epitope-administered animals.

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