Validation of a phage display and computational algorithm by mapping a conformational epitope of Bla g 2.

Title:

Validation of a phage display and computational algorithm by mapping a conformational epitope of Bla g 2.

identifier:	1931587
description:	epitope description:SMMKADFEEEPR host organism:Mus musculus antibody name:7C11
aggregation:	instance of dataset
availability:	available
primaryPublications:	22123204

study type:	b cell assays
subject species:

fullName:	Ruby Tiwari Surendra S Negi Benjamin Braun Werner Braun Anna Pomé s Martin D Chapman Randall M Goldblum Terumi Midoro-Horiuti

method:	antigen inhibition

name:	Validation of a phage display and computational algorithm by mapping a conformational epitope of Bla g 2.
description:	The phage displayed epitope inhibited the binding of Bla g 2 to anti-Bla g 2 mAb 7C11. MAb 7C11 was used to select the epitope from a random 12-mer phage display library by three rounds of panning. Phage displaying the epitope sequence or the same sequence with an Glu to Asp substitution at position 8 were the only clones selected. The authors mapped the epitope sequence to patches on the surface of the Bla g 2 structure centered on residues Pro61 and Arg83, which overlap with the epitope determined from the crystal structure.

name:	iedb
homePage:	http://www.iedb.org

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