Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1022990 |
| landingPage: |
http://www.iedb.org/assay/1931748 |
| type: |
Literature
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| publicationVenue: |
Transfusion
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| dates: |
2012
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| study type: | b cell assays |
| subject species: | Homo sapiens |
| fullName: |
Polina Obukhova
Elena Korchagina
Stephen Henry
Nicolai Bovin
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| method: |
ELISA
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| name: |
Natural anti-A and anti-B of the ABO system: allo- and autoantibodies have different epitope specificity.
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| description: |
The epitope is the terminal trisaccharide from blood group A antigen, and is shared among the three type A antigens.
Human Abs (IgG + IgM + IgA) from blood type B donors reacted with the epitope strongly, while a low but detectable reactivity was found in A blood type sera. Affinity purification of the epitope-specific Abs showed that the B-type alloantibodies do not cross-react with group A terminal minimal disaccharide and requires at least the epitope trisaccharide, while the A-type affinity purified epitope-specific auto-antibodies are reactive with the minimal disaccharide. This type of reactivity is shown to be of true auto-antibodies, not cross-reactive to B-type antigens, as it doesn't disappear when the serum was adsorbed to B-type trisaccharide antigen.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
