Neutralization of macrophage migration inhibitory factor (MIF) by fully human antibodies correlates with their specificity for the beta-sheet structure of MIF.

Title:

Neutralization of macrophage migration inhibitory factor (MIF) by fully human antibodies correlates with their specificity for the beta-sheet structure of MIF.

Dataset

identifier:	1938204
description:	epitope description:GFLSELTQQLAQATGKPP antigen name:Macrophage migration inhibitory factor host organism:Homo sapiens
aggregation:	instance of dataset
availability:	available
primaryPublications:	22238348

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1022899
landingPage:	http://www.iedb.org/assay/1938204

Publication

type:	Literature
publicationVenue:	J Biol Chem
dates:	2012

Dimension

study type:	b cell assays
subject species:	Homo sapiens

Person

fullName:	Randolf J Kerschbaumer Manfred Rieger Dirk Vö lkel Didier Le Roy Thierry Roger Jurate Garbaraviciene Wolf-Henning Boehncke Jü rgen Mü llberg Rene M Hoet Clive R Wood Gerhard Antoine Michael Thiele Helga Savidis-Dacho Michael Dockal Hartmut Ehrlich Thierry Calandra Friedrich Scheiflinger

Data Type

method:	ELISA

Study

name:	Neutralization of macrophage migration inhibitory factor (MIF) by fully human antibodies correlates with their specificity for the beta-sheet structure of MIF.
description:	The epitope sequence was deduced. MIF-specific antibodies were selected from the Dyax FAB310 phage display library using full-length MIF or six overlapping MIF fragments. The positive binders were expressed as human IgG4 antibodies. The epitope recognized by the mAb was deduced based on its reactivity with six overlapping MIF fragments.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on Feedback.

Related Publications

Feedback?