Immunological Data Discovery Index
Immunological Data Discovery Index
| Title: | The structural basis for serotype-specific neutralization of dengue virus by a human antibody. |
| identifier: | 1940210 |
| description: |
epitope description:T331, N332, Q411, Y412, L415, K416, G439, T440, T445, P446, Q447, E452, I453, L455, G554, T555, L588, K590, E664, K665
antigen name:Genome polyprotein
host organism:Homo sapiens
antibody name:HM14c10
|
| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
22723463 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1024472 |
| landingPage: |
http://www.iedb.org/assay/1940210 |
| type: |
Literature
|
| publicationVenue: |
Sci Transl Med
|
| dates: |
2012
|
| study type: | b cell assays |
| subject species: | Dengue virus 1 PVP159 |
| fullName: |
Ee Ping Teoh
Petra Kukkaro
En Wei Teo
Angeline P C Lim
Tze Tong Tan
Andy Yip
Wouter Schul
Myint Aung
Victor A Kostyuchenko
Yee Sin Leo
Soh Ha Chan
Kenneth G C Smith
Annie Hoi Yi Chan
Gang Zou
Eng Eong Ooi
D Michael Kemeny
Grace K Tan
Jowin K W Ng
Mah Lee Ng
Sylvie Alonso
Dale Fisher
Pei-Yong Shi
Brendon J Hanson
Shee-Mei Lok
Paul A MacAry
|
| method: |
electron microscopy
|
| name: |
The structural basis for serotype-specific neutralization of dengue virus by a human antibody.
|
| description: |
The epitope of Fab HM14c10 on Dengue virus 1 (strain PVP 159) was determined from the cryoelectron microscopy structure of the complex, solved to 7 angstroms (EMD-5268). There are 120 copies of Fab HM14c10 bound to all of the available 180 copies of envelope proteins on the virus surface. Two Fabs of HM14c10 bind to three envelope proteins in the virus asymmetric unit. Each antibody binds across two adjacent envelope proteins with half of the epitope on E-DIII and the other half on E-DI and the E-DI–E-DII hinge of a neighboring envelope protein. The binding footprints of the two HM14c10 Fabs in an asymmetric unit are not identical, with 12 amino acids common to both interfaces but 4 that are unique. (Epitope of Fab HM14c10 (I): T51, N52, L135, K136, G159, T160, T165, P166, Q167, E172, I173, G274, T275, L308, K310, E384, K385 and Fab HM14c10 (II): T51, N52, Q131, Y132, G159, T160, T165, P166, Q167, E172, I173, L175, G274, T275, L308, K310)
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| name: |
iedb
|
| homePage: |
http://www.iedb.org |