Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1014703 |
| landingPage: |
http://www.iedb.org/assay/1940878 |
| type: |
Literature
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| publicationVenue: |
J Immunol
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| dates: |
1993
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| study type: | b cell assays |
| subject species: | Homo sapiens |
| fullName: |
V R Bonagura
S E Artandi
A Davidson
I Randen
N Agostino
K Thompson
J B Natvig
S L Morrison
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| method: |
ELISA
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| name: |
Mapping studies reveal unique epitopes on IgG recognized by rheumatoid arthritis-derived monoclonal rheumatoid factors.
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| description: |
The heavy chain constant region of human IgG3 was linked to the variable heavy chain region of anti-dansyl murine mAb 27-44. The antibody antigen was expressed with a chimeric light chain made of human kappa constant region linked to the variable light chain region of the same murine mAb. The use of mutants and exon-shuffled chimeric IgG4 and IgG3 molecules allowed for the determination of the epitopic residue as involved in the recognition by a monoclonal Rheumatoid Factor (RF) antibody obtained from Rheumatoid Arthritis patients.
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| name: |
iedb
|
| homePage: |
http://www.iedb.org |
