Identification of epitopes on ADAMTS13 recognized by a panel of monoclonal antibodies with functional or non-functional effects on catalytic activity.

Title:

Identification of epitopes on ADAMTS13 recognized by a panel of monoclonal antibodies with functional or non-functional effects on catalytic activity.

Dataset

identifier:	1953994
description:	epitope description:QARGLLFSPAPQPRRLLPGPQEN antigen name:A disintegrin and metalloproteinase with thrombospondin motifs 13 host organism:Mus musculus antibody name:Pep4-5B-1
aggregation:	instance of dataset
availability:	available
primaryPublications:	22721582

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1025051
landingPage:	http://www.iedb.org/assay/1953994

Publication

type:	Literature
publicationVenue:	Thromb Res
dates:	2012

Dimension

study type:	b cell assays
subject species:	Homo sapiens

Person

fullName:	Atsuko Igari Terumichi Nakagawa Takanori Moriki Yusuke Yamaguchi Masanori Matsumoto Yoshihiro Fujimura Kenji Soejima Mitsuru Murata

Data Type

method:	biological activity

Study

name:	Identification of epitopes on ADAMTS13 recognized by a panel of monoclonal antibodies with functional or non-functional effects on catalytic activity.
description:	The epitope sequence was cited in Zheng et al. J. Biol. Chem. 276 (44), 41059-41063 (2001)[PMID:11557746]. The epitope sequence was deduced. The inhibitory effect of the epitope-specific monoclonal antibody was assessed by FRETS-VWF73 assay. Standard plasma of healthy donors was used as source of the antigen ADAMTS13. The epitope-specific mAb was able to inhibit VWF cleavage by ADAMTS13. The epitope was deduced based on reactivity to overlapping phage displayed peptide fragments.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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