Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1022205 |
| landingPage: |
http://www.iedb.org/assay/1954484 |
| type: |
Literature
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| publicationVenue: |
J Biol Chem
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| dates: |
2011
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| study type: | b cell assays |
| subject species: | Arabidopsis thaliana |
| fullName: |
Heidi Kaulfü
rst-Soboll
Stephan Rips
Hisashi Koiwa
Hiroyuki Kajiura
Kazuhito Fujiyama
Antje von Schaewen
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| method: |
western blot
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| name: |
Reduced immunogenicity of Arabidopsis hgl1 mutant N-glycans caused by altered accessibility of xylose and core fucose epitopes.
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| description: |
Protein extracts of Arabidopsis wild type and the N-glycosylation mutants were tested by Western-blot with the epitope-specific antibodies. The labeling of glycoproteins from mutants lacking Xylosyl transferase activity was markedly reduced compared to wild-type. Enzymatic treatment of A. thaliana protein extract with glycosidases removing residues different from Xylose also affected the recognition of the glycoprotein, implying the conformation and accessibility to recognition of the Xylose residue is affected by the presence other N-glycan residues.
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| name: |
iedb
|
| homePage: |
http://www.iedb.org |
