Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 1961125 |
| description: |
epitope description:H57, K58, D59, S60, H61, G62, R63, Q165, P166, E167, S168, G169, R170, R171
antigen name:Integrin alpha-IIb
host organism:Homo sapiens
antibody name:PA anti-alphaIIbß3 Ab
|
| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
22730538 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1024977 |
| landingPage: |
http://www.iedb.org/assay/1961125 |
| type: |
Literature
|
| publicationVenue: |
Blood
|
| dates: |
2012
|
| study type: | b cell assays |
| subject species: | Homo sapiens |
| fullName: |
Kazunobu Kiyomizu
Hirokazu Kashiwagi
Tsuyoshi Nakazawa
Seiji Tadokoro
Shigenori Honda
Yuzuru Kanakura
Yoshiaki Tomiyama
|
| method: |
flow cytometry
|
| name: |
Recognition of highly restricted regions in the beta-propeller domain of alphaIIb by platelet-associated anti-alphaIIbbeta3 autoantibodies in primary immune thrombocytopenia.
|
| description: |
The epitope residues recognized by the platelet-associated (PA) anti-alphaIIbß3 Abs from ITP patients were determined by analysis of binding to human-mouse chimera alphaIIbß3 molecules with small sections of the N-terminal half of the human molecule exchanged with the corresponding mouse sequence. In addition, mouse alphaIIbß3, with the human sequences W1:1-2 (HKDSHGR) and W2:3-4 (QPESGRR) exchanged in, was recognized similar to WT human alphaIIbß3 by PA anti-alphaIIbß3 Abs from the two patients. Single amino acid replacements S29K, R32S, E136Q and R139G reduced PA Ab binding, indicating these were critical residues.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
