Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1024977 |
| landingPage: |
http://www.iedb.org/assay/1961214 |
| type: |
Literature
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| publicationVenue: |
Blood
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| dates: |
2012
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| study type: | b cell assays |
| subject species: | Homo sapiens |
| fullName: |
Kazunobu Kiyomizu
Hirokazu Kashiwagi
Tsuyoshi Nakazawa
Seiji Tadokoro
Shigenori Honda
Yuzuru Kanakura
Yoshiaki Tomiyama
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| method: |
flow cytometry
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| name: |
Recognition of highly restricted regions in the beta-propeller domain of alphaIIb by platelet-associated anti-alphaIIbbeta3 autoantibodies in primary immune thrombocytopenia.
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| description: |
The epitope was deduced.
The epitope residues recognized by the platelet-associated (PA) anti-alphaIIbß3 Abs from ITP patients were determined by analysis of binding to human-mouse chimera alphaIIbß3 molecules with small sections of the N-terminal half of the human molecule exchanged with the corresponding mouse sequence. Exchange of the mouse sequence for the human epitope (W4:4-1) reduced binding of PA Abs from 4 of 15 ITP patients. In addition, mouse alphaIIbß3, with the human W4:4-1 sequence exchanged in, was recognized similar to WT human alphaIIbß3 by 3 of the 4 PA anti-alphaIIbß3 Abs.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
