Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.

Dataset

identifier:	1961245
description:	epitope description:N(6)-(glycylglycyl)-L-lysine residue host organism:Oryctolagus cuniculus antibody name:anti-diGly
aggregation:	instance of dataset
availability:	available
primaryPublications:	22871113

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1025081
landingPage:	http://www.iedb.org/assay/1961245

Publication

type:	Literature
publicationVenue:	J Proteome Res
dates:	2012

Dimension

study type:	b cell assays
subject species:

Person

fullName:	Chan Hyun Na Drew R Jones Yanling Yang Xusheng Wang Yanji Xu Junmin Peng

Data Type

method:	mass spectrometry

Study

name:	Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
description:	The epitope-specific mAb was used to isolate and identify trypsinized peptides with a diglycine tag on lysine residues in order to characterize the ubiquinated proteome in rat brain. The mAb was specific for K-GG (diglycine modified lysine residue) and did not react with linear GG peptides or pseudo K-GG peptides derived from iodoacetamide. A total of 1786 KGG sites on 2064 peptides in 921 proteins were identified.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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