Immunological Data Discovery Index
Immunological Data Discovery Index
| Title: | NMR structure and IgE epitopes of Blo t 21, a major dust mite allergen from Blomia tropicalis. |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1025148 |
| landingPage: |
http://www.iedb.org/assay/1958952 |
| type: |
Literature
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| publicationVenue: |
J Biol Chem
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| dates: |
2012
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| study type: | b cell assays |
| subject species: | Blomia tropicalis |
| fullName: |
Kang Wei Tan
Tan Ching Ong
Yun Feng Gao
Yuen Sung Tiong
Kang Ning Wong
Fook Tim Chew
Yu Keung Mok
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| method: |
ELISA
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| name: |
NMR structure and IgE epitopes of Blo t 21, a major dust mite allergen from Blomia tropicalis.
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| description: |
Epitopic residues recognized by serum IgE from allergic patients were identified by analysis of binding to Blo t 21 with single alanine substitution at each of 17 charged residues. Mutations that caused a >20% reduction in IgE binding in more than 50% of the patients' sera were: E89A (11 of 12 patients), D79A (8 of 12), D96A (7 of 12), K107A (7 of 12), and E84A (6 of 12). Mutation E74A reduced IgE binding in 4 of 12 patient sera and was also identified as an IgE epitope in Blo t 5. IgE binding was further reduced to Blo t 21 containing mutations at two to four of the residues E74, D79, E89, and D96.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |