Epitope mapping by random peptide phage display reveals essential residues for vaccinia extracellular enveloped virion spread.

Title:

Epitope mapping by random peptide phage display reveals essential residues for vaccinia extracellular enveloped virion spread.

identifier:	1962418
description:	epitope description:ACCDDWPHEC host organism:Mus musculus antibody name:1G10
aggregation:	instance of dataset
availability:	available
primaryPublications:	23006741

study type:	b cell assays
subject species:

fullName:	Yong He Yonggang Wang Evi B Struble Pei Zhang Soma Chowdhury Jennifer L Reed Michael Kennedy Dorothy E Scott Robert W Fisher

method:	phage display

name:	Epitope mapping by random peptide phage display reveals essential residues for vaccinia extracellular enveloped virion spread.
description:	The epitope is a mimotope of the vaccinia virus A33 protein (EEV). MAb 1G10 was used to screen a disulfide-constrained phage library in which linear peptides were present. In this approach, the randomized peptide segment is flanked by paired cysteines, which are oxidized during phage assembly to present the peptide as a taut loop at the N-terminus of the minor phage coat protein PIII.

name:	iedb
homePage:	http://www.iedb.org

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