Amino acid residue-specific neutralization and nonneutralization of hepatitis C virus by monoclonal antibodies to the E2 protein.

Title:

Amino acid residue-specific neutralization and nonneutralization of hepatitis C virus by monoclonal antibodies to the E2 protein.

identifier:	1963444
description:	epitope description:NTGWLAGLFYQHK antigen name:Genome polyprotein host organism:Mus musculus BALB/c antibody name:MAbs #8, #12, #41, #50
aggregation:	instance of dataset
availability:	available
primaryPublications:	22973024

study type:	b cell assays
subject species:	Hepatitis C virus (isolate H77)

fullName:	Hongying Duan Alla Kachko Lilin Zhong Evi Struble Shivani Pandey Hailing Yan Christine Harman Maria Luisa Virata-Theimer Lu Deng Zhong Zhao Marian Major Stephen Feinstone Pei Zhang

method:	ELISA

name:	Amino acid residue-specific neutralization and nonneutralization of hepatitis C virus by monoclonal antibodies to the E2 protein.
description:	From mice immunized with the fragment E2(412-447), 4 out of 7 mAbs were directed to the epitope, located at the N-terminus of the immunizing peptide. From random peptide library panning and alanine scanning, the residues required for binding were found to be W437 and L438 for the neutralizing mAbs #8 and #41, and W437, L438 and L441 for the non-neutralizing mAbs #12 and #50, and for the latter also residue F442 showed involvement.

name:	iedb
homePage:	http://www.iedb.org

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on Feedback.