Epitope structure and binding affinity of single chain llama anti-beta-amyloid antibodies revealed by proteolytic excision affinity-mass spectrometry.

Title:

Epitope structure and binding affinity of single chain llama anti-beta-amyloid antibodies revealed by proteolytic excision affinity-mass spectrometry.

Dataset

identifier:	1966803
description:	epitope description:LVFFAEDVGSNK antigen name:Amyloid beta A4 protein host organism:Homo sapiens antibody name:Nb_3, Nb_9
aggregation:	instance of dataset
availability:	available
primaryPublications:	23280612

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1025388
landingPage:	http://www.iedb.org/assay/1966803

Publication

type:	Literature
publicationVenue:	J Mol Recognit
dates:	2013

Dimension

study type:	b cell assays
subject species:	Homo sapiens

Person

fullName:	Gabriela Paraschiv Cé cile Vincke Paulina Czaplewska Marilena Manea Serge Muyldermans Michael Przybylski

Data Type

method:	mass spectrometry

Study

name:	Epitope structure and binding affinity of single chain llama anti-beta-amyloid antibodies revealed by proteolytic excision affinity-mass spectrometry.
description:	Affinity-mass spectrometric characterization of Ab(1–40) was carried out. Proteolytic digestion with trypsin. chymotrypsin, GluC, or LysC of 10 mg A beta (1–40) was performed and the the proteolytic digest mixture was then applied to a column containing the epitope-specific nanobody.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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