Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 1966706 |
| description: |
epitope description:beta-D-QuiN4Fm-(1->4)-alpha-D-GalNAcAN-(1->4)-alpha-D-GalNAcAN-(1->3)-beta-D-QuiNAc-(1->4)-[alpha-D-GalN-(1->2)]-[alpha-D-Glc-(1->3)]-beta-D-Man-(1->4)-[beta-D-Glc-(1->2)]-alpha-D-Man-(1->5)-alpha-Kdo
antigen name:lipopolysaccharide
host organism:Mus musculus BALB/c
antibody name:Ab52
|
| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
22747335 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1024890 |
| landingPage: |
http://www.iedb.org/assay/1966706 |
| type: |
Literature
|
| publicationVenue: |
Biochemistry
|
| dates: |
2012
|
| study type: | b cell assays |
| subject species: | Francisella tularensis |
| fullName: |
Michael J Rynkiewicz
Zhaohua Lu
Julia H Hui
Jacqueline Sharon
Barbara A Seaton
|
| method: |
x-ray crystallography
|
| name: |
Structural analysis of a protective epitope of the Francisella tularensis O-polysaccharide.
|
| description: |
The X-ray crystal structure of the unliganded epitope-specific Fab Ab52 was determined to 2.1 Å by molecular replacement [PBD 3UJT]. An antibody−epitope (two tetrasaccharide repeats, ABCDA′B′C′D') complex was derived using molecular docking. The docked complex shows a V-shaped epitope encompassing six of the eight sugar residues, alpha-D-GalpNAcAN(1→4)-alpha-D-GalpNAcAN(1→3)-beta-D-QuipNAc(1→2)-beta-D-Quip4NFm-(1→4)-alpha-D-GalpNAcAN(1→4)-alpha-D-GalpNAcAN (BCDA′B′C′), with the central disaccharide, at the point of the V, buried in a pocket deep inside the center of the floor of a large canyon-shaped Ab52 binding site.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
