The core cysteines, (C909) of islet antigen-2 and (C945) of islet antigen-2beta, are crucial to autoantibody binding in type 1 diabetes.

Title:

The core cysteines, (C909) of islet antigen-2 and (C945) of islet antigen-2beta, are crucial to autoantibody binding in type 1 diabetes.

identifier:	1967173
description:	epitope description:W799, C909 antigen name:Receptor-type tyrosine-protein phosphatase-like N host organism:Homo sapiens
aggregation:	instance of dataset
availability:	available
primaryPublications:	22966073

study type:	b cell assays
subject species:	Homo sapiens

fullName:	Karen T Elvers Ivey Geoghegan Debbie K Shoemark Vito Lampasona Polly J Bingley Alistair J K Williams

method:	radio immuno assay (RIA)

name:	The core cysteines, (C909) of islet antigen-2 and (C945) of islet antigen-2beta, are crucial to autoantibody binding in type 1 diabetes.
description:	The epitope was derived from the author provided GenBank identifier L18983 (GI:499630). Mutational analysis of the PTP IA-2 region revealed a significant reduction of binding of T1D patient sera when residue W799 and C909 were substituted with alanine or serine, respectively. This suggests that these residues are critical for binding. Pretreatment with N-ethylmaleimide caused little additional reduction of binding by these sera. Interestingly, serine substitutions at C623, C717, C726 and C820 caused modest increases in binding of patient sera.

name:	iedb
homePage:	http://www.iedb.org

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