Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1025422 |
| landingPage: |
http://www.iedb.org/assay/1968269 |
| type: |
Literature
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| publicationVenue: |
Anal Chem
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| dates: |
2013
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| study type: | b cell assays |
| subject species: |
| fullName: |
Stephen Cunningham
Emily Starr
Iain Shaw
John Glavin
Marian Kane
Lokesh Joshi
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| method: |
antigen inhibition (~ IC50)
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| name: |
Development of a convenient competitive ELISA for the detection of the free and protein-bound nonhuman galactosyl-alpha-(1,3)-galactose epitope based on highly specific chicken single-chain antibody variable-region fragments.
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| description: |
Following the second round of panning, the presence of an enriched population of phage-displayed scFvs demonstrating binding to Gal-alpha-(1,3)-Gal-BSA was confirmed by direct ELISA. From 74 random picked positive clones, 6 were sequenced and 4 unique sequences were isolated. Clone A4 was expressed as soluble scFvs and confirmed by direct and competitive ELISA to bind specifically to the epitope, and minimally to other carbohydrates bound to BSA or HSA, except for those containing the epitope. The scFv was used to detect and quantify the epitope content in several glycoproteins.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
