Development of a convenient competitive ELISA for the detection of the free and protein-bound nonhuman galactosyl-alpha-(1,3)-galactose epitope based on highly specific chicken single-chain antibody variable-region fragments.

Title:

Dataset

identifier:	1968272
description:	epitope description:alpha-D-galactosyl-(1->3)-D-galactose host organism:Gallus gallus Leghorn antibody name:A4
aggregation:	instance of dataset
availability:	available
primaryPublications:	23215249

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1025422
landingPage:	http://www.iedb.org/assay/1968272

Publication

type:	Literature
publicationVenue:	Anal Chem
dates:	2013

Dimension

study type:	b cell assays
subject species:

Person

fullName:	Stephen Cunningham Emily Starr Iain Shaw John Glavin Marian Kane Lokesh Joshi

Data Type

method:	antigen inhibition (~ IC50)

Study

name:	Development of a convenient competitive ELISA for the detection of the free and protein-bound nonhuman galactosyl-alpha-(1,3)-galactose epitope based on highly specific chicken single-chain antibody variable-region fragments.
description:	The epitope-specific scFv fragment was shown to react with several compounds containing the epitope in competitive and direct ELISA, with lower IC50 values than to the epitope, suggesting that it had a higher affinity for the larger sugars than for the disaccharide.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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