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identifier: 1968797
description:
epitope description:L8, T9, Q31, D32, K33, E34, G35, I36, P37, Q40, R42, L71, L73, R74, G75, G76, M77, P95, S133, N136, I137, Q138, K139
antigen name:Polyubiquitin-C (UniProt:P0CG48)
host organism:Homo sapiens
antibody name:1F11/3F5/Y102L
aggregation:
instance of dataset
availability:
available
primaryPublications: 22227388
authorizations:
registration not required
accessURL: http://www.iedb.org/reference/1025437
landingPage: http://www.iedb.org/assay/1968797
type:
Literature
publicationVenue:
J Mol Biol
dates:
2012
study type: b cell assays
subject species: Homo sapiens
fullName:
Marissa L Matsumoto
Ken C Dong
Christine Yu
Lilian Phu
Xinxin Gao
Rami N Hannoush
Sarah G Hymowitz
Donald S Kirkpatrick
Vishva M Dixit
Robert F Kelley
method:
western blot
name:
Engineering and structural characterization of a linear polyubiquitin-specific antibody.
description:
Epitope residues were calculated from [PDB: 3U30] as the antigen residues at 4Å
distance from the antibody.
The epitope-specific mAb 1F11/3F5/Y102L recognized linear diubiquitin (diUb) on Western blots, and did not react with monoUb, K6-linked, K11-linked, K27-linked, K29-linked, K33-linked, K48-linked, or K63-linked diUbs.

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